Two days of Educational Short Course Offerings
Two days of Educational Short Courses will be offered the Saturday and Sunday preceding the conference. The courses are taught by leading academic and industrial scientists and cover both fundamentals as well as real-world application examples. Certificates of completion recognizing attendance will be awarded. HPLC 2014 New Orleans reserves the right, without notice, to modify the short course material or schedules, as well as to amend the roster of short course presenters. Short courses are open to conference and non-conference participants. Short course registration is either within a bundled registration fee package or may be purchased separately. Fees are posted at http://hplc2014.org/reg.html. The final deadline to register for short courses is extended to April 30 or until the course is sold out. Short course notes will be emailed to each course participant to download the course notes. Each short course participant should bring a copy of the short course notes with them to the short course. There will be no printed copies available.
Short Course 1: Chiral Separations
Instructors: Daniel Armstrong and Zachary Breitbach, University of Texas, Arlington
Saturday, May 10 [9:00am-12:00pm]
Room: Oak Alley, 3rd floor of Hilton
This introductory short course is designed for researchers faced with separation or analysis of enantiomeric compounds. The goal of the course is to familiarize the participants with the best available options when confronted with the need for chiral separations. The first part of the material to be covered will include a review of the terminology of chirality and a brief overview of the theory behind enantiomeric separations. In addition, new products and SFC techniques will be discussed. Participants should have a basic knowledge of chromatography and have previous experience with routine HPLC (reverse phase and normal phase). Course will include: history, background and nomenclature; summary of LC techniques; new chiral selectors and method development; supercritcal fluid (SFC) separations; and discussion and questions.
About the Instructors:
Dr. Daniel W. Armstrong is the Robert A. Welch Professor of Chemistry at the University of Texas at Arlington. He has over 550 publications including 29 book chapters, one book (“Use of Ordered Media in Chemical Separations”) and 23 patents. He is considered the “Father” of pseudophase (micelle and cyclodextrin-based) separations. He elucidated the first chiral recognition mechanism for cyclodextrins. He also first developed macrocyclic antibiotics and cyclofructans as chiral selectors. He is one of the world’s leading authorities on the theory, mechanism and use of enantioselective molecular interactions. He also developed the most comprehensive solvation and characterization models for room temperature ionic liquids (RTILs) and pioneered their use in analytical chemistry, particularly as new GC stationary phases recently commercialized by Supelco. He also developed ILs as the most advance MALDI-MS matrices and as LC & CE EST-MS ion pairing agents (PIESI) for trace detection of anions in the positive mode.
Dr. Zachary Breitbach obtained his Bachelor of Science degree in chemistry from Clarke College in 2005. He received his Doctor of Philosophy degree in analytical chemistry from The University of Texas at Arlington under the direction of Professor Daniel Armstrong in 2010. He continued his career at UTA serving as a Research Scientist where he helped develop new chiral stationary phases for HPLC, SFC and GC. He also serves as a consultant to AZYP, LLC regarding new chemical separation media focused on chiral and HILIC HPLC separations. He has published approximately 40 peer-reviewed research manuscripts, holds 1 patent, and has presented work at numerous research conferences.
Short Course 2: Ionic Liquids in Separations
Instructors: Daniel Armstrong, University of Texas, Arlington; Jared Anderson, University of Toledo
Saturday, May 10 [1:00pm-4:00pm]
Room: Oak Alley, 3rd floor of Hilton
Ionic liquids (ILs) are having a significant impact on the way we do chemical analysis. ILs have outstanding thermal properties, unique selectivities and are resistant to water, oxygen and other species that react with or decompose molecular solvents. This course provides an introduction to the structure and properties of ILs that make them unique entities that are useful in chemical analysis. This half day course will have four lectures and a question and answer period as follows: (1) Structure properties and classification of ILs. (2) Ionic liquids as solvents in analytical extraction and microextractions. (3) ILs in other separations, µTAS and mass spectrometry. (4) Use of ILs and polymeric ILs in multidimensional chromatography and miscellaneous techniques. (5) Questions and answers.
About the Instructors:
Dr. Daniel W. Armstrong is the Robert A. Welch Professor of Chemistry at the University of Texas at Arlington. He has over 550 publications including 29 book chapters, one book (“Use of Ordered Media in Chemical Separations”) and 23 patents. He also developed the most comprehensive solvation and characterization models for room temperature ionic liquids (RTILs) and pioneered their use in analytical chemistry, particularly as new GC stationary phases recently commercialized by Supelco. He also developed ILs as the most advance MALDI-MS matrices and as LC & CE EST-MS ion pairing agents (PIESI) for trace detection of anions in the positive mode. He is considered the “Father” of pseudophase (micelle and cyclodextrin-based) separations. He elucidated the first chiral recognition mechanism for cyclodextrins. He also first developed macrocyclic antibiotics and cyclofructans as chiral selectors. He is one of the world’s leading authorities on the theory, mechanism and use of enantioselective molecular interactions.
Dr. Jared L. Anderson obtained his Bachelor of Science degree in chemistry at South Dakota State University in 2000 and his Ph.D. in Analytical Chemistry at Iowa State University in 2005. He joined The University of Toledo in 2005 as an Assistant Professor of Chemistry and was promoted to Associate Professor of Chemistry with tenure in 2009. In 2011, he was promoted to the rank of Full Professor of Chemistry. He has over 85 publications including 5 book chapters and holds 5 patents. His research interests include the synthesis of new classes of ionic liquids and materials derived from polymeric ionic liquids, the use of ionic liquids as catalytic solvents, and all aspects of separation science including sample preparation and chromatography.
Short Course 3: LC/MS Method Development for Small Molecule Pharmaceuticals
Instructor: Perry Wang, U.S. Food and Drug Administration
Saturday, May 10 [9:00am-4:00pm]
Room: Belle Chasse, 3rd floor of Hilton
This course offers practical training for the practical scientists. It will take the participants step-by-step through the concepts and techniques to develop LC-MS methods. The emphasis is on practical issues associated with developing LC-MS method for small molecule pharmaceuticals. It also emphasizes problem-solving skills with examples encountered in the pharmaceutical industry and academic research. This course will provide the participants with an updated overview and a solid working knowledge of LC-MS. The participants will learn useful theoretical concepts, instrumental fundamentals and operating principles, column basics and selection guide, and key applications in the pharmaceutical industry and other field. After this course, the participants will be able to independently develop their LC-MS methods for small molecules. New technologies and techniques, such as monolithic chromatography and hydrophilic interaction liquid chromatography (HILIC) will be presented. Since the pharmaceutical industry is regulated by GLP and GMP, some regulation and validation concepts will be introduced. This one-day course is intended for analysts, lab managers, regulators and researchers using LC-MS in the analytical laboratories. It will benefit the scientists ranging from college students to professionals in the fields of the pharmaceutical industry and other analytical fields.
About the Instructor:
Dr. Perry G. Wang is a research chemist in the Office of Regulatory Science, US Food and Drug Administration (FDA). His interests include analytical method development and validation for drugs and constituents of foods and cosmetic products using advanced instrumentation. His expertise focuses on high throughput drug analysis by LC-MS/MS. His current research focuses on developing analytical methods for constituents in cosmetics and dietary supplements by GC-MS/MS and LC-MS/MS. He received his B.S. degree in chemistry from Shandong University, China and Ph.D. degree in environmental engineering from Oregon State University, USA.
Short Course 4: HPLC and UHPLC Troubleshooting: A Performance Qualification Approach
Instructor: John Dolan, LC Resources
Saturday, May 10 [9:00am-4:00pm]
Room: Jasperwood, 3rd floor of Hilton
A comprehensive short course in the isolation, correction, and prevention of liquid chromatographic problems. This course is for anyone who works with HPLC or UHPLC. No previous experience is assumed; however, much of the course will appeal to the more experienced worker. This one-day course teaches how to solve problems with your LC methods, how to qualify HPLC performance using specific tests that also can be used for problem isolation, and how to prevent many problems from happening. Topics include: Operating principles of each module in an HPLC and UHPLC system; Review of HPLC theory as it applies to troubleshooting and instrument maintenance; Why performance qualification (PQ) is so important for reliable operation of your HPLC and UHPLC and ensuring the quality of data; and Systematic techniques for problem-solving and instrument maintenance. Lecture topics: Section 1. Principles of HPLC & UHPLC Troubleshooting. Section 2. Performance Qualification, Part 1: Pump & Detector. Section 3. PQ, Part 2: On-Line Mixing. Section 4. PQ, Part 3: Chromatographic Checks. Section 5. The Separation: Physical Problems with Columns. Section 6. The Separation: Chemical Problems with Columns. Section 7. Problems with Quantification.
About the Instructor:
John Dolan is well known as one of the world’s experts in HPLC. His “LC Troubleshooting” column has appeared monthly in LCGC for over 30 years. His three books and more than 100 technical papers on HPLC testify to his wide range of expertise in HPLC-related areas. Through LC Resources he has provided HPLC training classes to more than 10,000 practitioners world-wide, appearing regularly as a short-course provider at the annual HPLC meetings.
Short Course 5: Drug Quality Fundamentals: QC of Small Molecule Drugs and Recombinant Biologics
Instructor: Michael Dong, Genentech
Sunday, May 11 [9:00am-12:00pm]
Room: Oak Alley, 3rd floor of Hilton
This course provides an overview of drug quality concepts, standards, regulations, and practices in clinical development and manufacturing in pharmaceutical and biotechnology industries. You will learn the roles of quality assurance (QA), quality control (QC), and the chemistry, manufacturing, and control (CMC) drug development process in ensuring safety, efficacy and quality of drug products. It covers industry-standard processes such as qualification/validation, release testing, specifications, certificates of analysis (COA), stability and regulatory filing. Critical quality attributes (CQA) of large-molecule recombinant biologics are described and contrasted with those of small molecule drugs. The course discusses the challenges of maintaining drug quality in a global supply chain and the role of HPLC in these processes. Three major agenda topics are: (1) Introduction to Drug Quality and CMC Quality Assurance Process: quality attributes, recent quality breaches, GLP, GMP, GCP, ICH guidelines, IND, NDA, CTA, QC / QA unit, specifications, and Quality by Design (QbD). (2) QC of Small Molecule Drug Substance and Drug Product: specifications, release testing, COA, impurities and stability. (3) Critical Quality Attributes of Recombinant Biological Products: approaches to manufacturing and bioprocessing of monoclonal antibodies, analysis of intact proteins, fragments, variants, glycans and other biological testing.
About the Instructor:
Dr. Michael W. Dong is a Senior Scientist at Genentech, Small Molecule Analytical Chemistry and Quality Control, CA. He was Research Fellow at Purdue Pharma and Sr. Staff Scientist at Applied Biosystems / PerkinElmer. He holds a Ph.D. in Analytical Chemistry from City University of New York and has over ten years of experience in drug development. He has conducted training courses on HPLC/UHPLC, drug development process and drug quality fundamentals at national meetings. He has authored 3 books and 100+ publications in analytical chemistry including a best-seller in chromatography – Modern HPLC for Practicing Scientists, Wiley.
Short Course 6: Two-dimensional Liquid Chromatography: Principles, Instrumentation, Method Development, and Applications
Instructors: Mark Schure, Kroungold Analytical; Martin Gilar, Waters; Rob Murphy, Kroungold Analytical
Sunday, May 11 [9:00am-4:00pm]
Room: Belle Chasse, 3rd floor of Hilton
In 2DLC, sample components are fractionated by two different columns utilizing different retention mechanisms. To achieve successful 2D resolution of complex sample components, dissimilar (orthogonal) retention mechanisms are required to effectively spread the peaks throughout the available separation space. In this course we will discuss in detail: Theory of 2DLC, from a practical point of view; Instrumentation; Method development ; and Applications to peptides, proteins, industrial polymers, and surfactants. Students are expected to be familiar with HPLC. Those who take this course will learn background information essential to understanding the technique and achieve practical results with commercial instrumentation. Many aspects of 2DLC are shared with one-dimensional HPLC such as column technologies, pumps, solvent systems, and matching the detector. However, 2DLC has some issues which are not present in one-dimensional HPLC, and these will be explained in detail so that course participants will have this knowledge prior to starting method development. We will also explore the suitable instrumentation for 2DLC, and how to process data external to the acquisition software. Applications of comprehensive 2DLC will be shown for complex industrial and biological samples, as well as simple applications such as column switching, target peak purity investigation, and biopolymer analysis using commercial two-dimensional chromatographic instruments.
About the Instructors:
Dr. Martin Gilar is a principal investigator in Core Research group at Waters Corporation. He has more than 20 years of experience in the separation sciences, including chromatography, electrophoresis, and mass spectrometry. His research interest is analysis of biopolymers, and 2D LC. He has published over 40 peer reviewed papers. Dr. Gilar’s received his Ph.D. in analytical chemistry from Institute of Chemical Technology in Prague (1996). He spent postdoc years in Hybridon Inc. (1996-1998) and Northeastern University in Boston (1998) developing separation methods for antisense oligonucleotides and fraction collector for DNA molecules. Since 1998 he works at Waters Corp. in Milford, Massachusetts.
Dr. Robert E. Murphy is a consultant at Kroungold Analytical Inc. He is an analytical chemist with 30 years of separation science and MS experience in the natural products, polymer, instrument, and biotech industries. He has published 13 peer reviewed journal articles and 4 book chapters, with emphasis on developing separation systems for the analysis of complex samples. He currently consults on the use of LC/MS and two-dimensional liquid chromatography combined with high resolution MS for the analysis of biomolecules, biomarkers, and other difficult samples in the academic, instrument, biotech, and contract research organizations.
Dr. Mark Schure is Chief Technology Officer of Kroungold Analytical, a consulting company which specializes in new product development for separation science. In addition, Dr. Schure has been an Adjunct Professor of Chemical Engineering at the University of Delaware since 1995. He was chairman of the HPLC 2004 conference in Philadelphia and has been co-chairman of the ISPPP conference series in the United States. He has published over 100 papers, 4 patents and recently edited the book “Multidimensional Liquid Chromatography.” His contributions to separation science include 2D chromatography, chromatographic mechanism(s), capillary electrophoresis and field-flow fractionation utilizing theory, simulation and experimental investigation.
Short Course 7: Capillary Electrophoresis for Biomolecules (CANCELLED)
Instructor: Robert Weinberger, CE Technologies
Sunday, May 11 [9:00am-4:00pm]
Room: Jasperwood, 3rd floor of Hilton
In this specially designed 1 day course, you will learn how to develop methods and implement CE for the separation of biomolecules: primarily proteins, glycoproteins, peptides and carbohydrates. The course begins with a review describing the factors that influence the electrophoretic migration of molecules in fluid solution. A phenomenological approach is employed that enables students to take home a thorough understanding of electrophoretic processes. The techniques employed are capillary zone electrophoresis (CZE), capillary isoelectic focusing (CIEF) capillary sieving electrophoresis (CSE) for size separations and micellar electrokinetic chromatography (MEKC). Microfabricated systems will be briefly reviewed. The course is designed for chemists, biochemists and those in related fields who wish to implement Capillary Electrophoresis in their laboratories. Both commercial kits and user developed methods will be described. Good operating practices to maximize repeatability are extensively covered as is troubleshooting. Students will have the opportunity to share their non-confidential separations with the class. Bring your separations on flash drives using PowerPoint. Students are encouraged to contact the instructor in advance and describe their separation needs.
About the Instructor:
Dr. Robert Weinberger is a consultant specializing in the field of Capillary Electrophoresis (CE). Since 1991 he has been the principal of CE Technologies, Inc., a consulting firm specializing in contract research, methods developmenand short courses. His column on CE appeared often in American Laboratory and he is the author of 50 technical publications, patents, books and book chapters as well as 100 magazine articles and meeting reviews. He served as an expert witness in CE patent litigation surrounding DNA sequencing and offered expert opinions and testimony concerning the purity of heparin samples, adulterated drugs and counterfeited nutraceuticals. Over the years, he has produced hundreds of separations covering large and small molecules across many different industries.